Preparation of 0.05M Disodium edetate or EDTA

Preparation of 0.05M Disodium edetate or EDTA
Molecular Formula:  C10H14N2Na2O8·2H2O			Molecular weight: 372.24
Preparation	:	Dissolve 18.6 g of edetate disodium transfer into a 1000ml volumetric flask then volume up to 1000 ml with water.
Standardization	:	chemometric standard :   Step-01: Dry Calcium Carbonate At first, dry calcium carbonate at 110 for 2 hours and cool in a desiccator.   Step-02: Procedure Accurately weigh about 200 mg of calcium carbonate transfer into a 250-ml conical flask, then 5 mL of diluted hydrochloric acid (3.0 N hydrochloric acid) from a pipet inserted between the lip of the beaker and the edge of the watch glass. and dilute with water to about 100 mL. While stirring the solution, preferably with a magnetic stirrer, add about 30 mL of the edetate disodium solution from a 50-mL buret. Add 15 mL of sodium hydroxide TS and 300 mg of hydroxy naphthol blue (an indicator), and continue the titration with the edetate disodium solution to a blue endpoint.
Calculation	:	M = (gCaCO3)(1000) 100.09 x ml EDTA
Reference	:	USP